ELK1 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with ELK1 monoclonal antibody, unconjugated (bsm-61173R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ELK1 monoclonal antibody, unconjugated (bsm-61173R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IF(ICC)
IP

Reactivity

Human
Mouse

Predicted Reactivity

Overview
Catalog #	bsm-61173r-TR
Product Name	ELK1 Recombinant Antibody
Applications	WB IF(ICC) IP
Reactivity	Human, Mouse
Predicted Reactivity	Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ELK1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2002
Swiss Prot	P19419
Subcellular location	Nucleus
Synonyms	ELK1; ETS-domain protein ELK-1; ETS-domain protein Elk-1.
Background	Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK and MAPK-signaling pathways stimulation.
Application Dilution
WB	1:500-2000
IF(ICC)	IFICC1:50-200
IP	IP1:20-50