ELK1 Recombinant Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61173r-percp-Cy5.5-100uL |
| Product Name | ELK1 Recombinant Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ELK1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2002 |
| Swiss Prot | P19419 |
| Subcellular location | Nucleus |
| Synonyms | ELK1; ETS-domain protein ELK-1; ETS-domain protein Elk-1. |
| Background | Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK and MAPK-signaling pathways stimulation. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is ELK1 Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-61173R-PERCP-CY5-5) validated for, and at what dilution?
BSM-61173R-PERCP-CY5-5 is validated for WB(WB) (1:500-2000); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ELK1 Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-61173R-PERCP-CY5-5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should BSM-61173R-PERCP-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ELK1 Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-61173R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human ELK1
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BSM-61173R-PERCP-CY5-5)?
PerCP-Cy5.5 on BSM-61173R-PERCP-CY5-5: excitation / emission 482nm/694nm.