Bak Recombinant Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61174r-apc |
| Product Name | Bak Recombinant Antibody, APC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BAK1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | BAK1;BAK;BAK-LIKE;BCL2L7;CDN1. |
| Background | In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1ug/Test |
| IF(IHC-P) | IFIHC-P1:200-500 |
| IF(ICC) | IFICC1:100-500 |
Frequently asked questions
Which applications is Bak Recombinant Antibody, APC Conjugated (bsm-61174R-APC) validated for, and at what dilution?
bsm-61174R-APC is validated for WB(WB) (1:500-2000); FCM(FCM) (1ug/Test); IF(IHC-P)(IF(IHC-P)) (1:200-500); IF(ICC)(IF(ICC)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Bak Recombinant Antibody, APC Conjugated (bsm-61174R-APC) react with?
Tested reactivity: Human.
How should bsm-61174R-APC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Bak Recombinant Antibody, APC Conjugated (bsm-61174R-APC)?
KLH conjugated synthetic peptide derived from human BAK1
What are the excitation and emission wavelengths of the APC conjugate (bsm-61174R-APC)?
APC on bsm-61174R-APC: excitation / emission 650nm/660nm.