HSP60 Recombinant Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61183r-bf750 |
| Product Name | HSP60 Recombinant Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Hsp60 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3329 |
| Swiss Prot | P10809 |
| Subcellular location | Cytoplasm |
| Synonyms | HSPD1;CPN60;GROEL;HLD4;HSP60;HSP65;HuCHA60;SPG13. |
| Background | Chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp10, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. |
| Application Dilution | |
| WB | 1:1000-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:100-200 |
Frequently asked questions
Which applications is HSP60 Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-61183R-BF750) validated for, and at what dilution?
bsm-61183R-BF750 is validated for WB(WB) (1:1000-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HSP60 Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-61183R-BF750) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61183R-BF750 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HSP60 Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-61183R-BF750)?
KLH conjugated synthetic peptide derived from human Hsp60
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bsm-61183R-BF750)?
AbBy Fluor-750 on bsm-61183R-BF750: excitation / emission 749nm/775nm.