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Hsc70 Recombinant Antibody, APC Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61188r-apc-100uL
Product Name Hsc70 Recombinant Antibody, APC Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Hsc70
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3312
Swiss Prot P11142
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms Heat shock cognate 71 kDa protein; HS7C; HSP73; HSP7C; HSPA10; HSPA8.
Background Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, chaperone-mediated autophagy, activation of proteolysis of misfolded proteins, formation and dissociation of protein complexes, and antigen presentation. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:50-200
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is Hsc70 Recombinant Antibody, APC Conjugated (bsm-61188R-APC) validated for, and at what dilution?

bsm-61188R-APC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Hsc70 Recombinant Antibody, APC Conjugated (bsm-61188R-APC) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61188R-APC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Hsc70 Recombinant Antibody, APC Conjugated (bsm-61188R-APC)?

KLH conjugated synthetic peptide derived from human Hsc70

What are the excitation and emission wavelengths of the APC conjugate (bsm-61188R-APC)?

APC on bsm-61188R-APC: excitation / emission 650nm/660nm.