BBC3/PUMA Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61191r-bf647-100uL |
| Product Name | BBC3/PUMA Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BBC3 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27113 |
| Swiss Prot | Q9BXH1 |
| Subcellular location | Cytoplasm |
| Synonyms | BBC3; bcl2 binding component 3; jfy1; puma; p53 up-regulated modulator of apoptosis; PUMA alpha. |
| Background | Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis.Promotes partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53, releasing the bound p53/TP53 to induce apoptosis. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is BBC3/PUMA Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61191R-BF647) validated for, and at what dilution?
bsm-61191R-BF647 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BBC3/PUMA Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61191R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61191R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BBC3/PUMA Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61191R-BF647)?
KLH conjugated synthetic peptide derived from human BBC3
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-61191R-BF647)?
AbBy Fluor-647 on bsm-61191R-BF647: excitation / emission 650nm/665nm.