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YB1 Recombinant Antibody, AbBy Fluor-488 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61192r-bf488-100uL
Product Name YB1 Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-488
Host Rabbit
Source KLH conjugated synthetic peptide derived from human YBX1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4904
Swiss Prot P67809
Subcellular location Cytoplasm, Nucleus, Secreted
Synonyms CBF-A; DBPB; NSEP1; p50; YB1; YBX1; DNA binding protein B.
Background DNA- and RNA-binding protein involved in various processes, such as translational repression, RNA stabilization, mRNA splicing, DNA repair and transcription regulation.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is YB1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61192R-BF488) validated for, and at what dilution?

bsm-61192R-BF488 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does YB1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61192R-BF488) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61192R-BF488 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise YB1 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61192R-BF488)?

KLH conjugated synthetic peptide derived from human YBX1

What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-61192R-BF488)?

AbBy Fluor-488 on bsm-61192R-BF488: excitation / emission 499nm/519nm.