Phospho-Histone H2B.1 (Thr129) Recombinant Antibody

$Western blot analysis of Saccharomyces cerevisiae cell lysate treated with Methyl methanesulfonate. Using Phospho-Histone H2B.1 (Thr129) (bsm-61195R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of Saccharomyces cerevisiae cell lysate treated with Methyl methanesulfonate. Using Phospho-Histone H2B.1 (Thr129) (bsm-61195R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

Reactivity

Human
Yeast

Overview
Catalog #	bsm-61195r
Product Name	Phospho-Histone H2B.1 (Thr129) Recombinant Antibody
Applications	WB
Reactivity	Human, Yeast
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from yeast Histone H2B.1 around the phosphorylation site of T129
Modification Site	Thr129
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	851810
Swiss Prot	P02293
Synonyms	HTB2; Htb2p; HTB1; Htb1p; Histone H2B.1; Histone H2B.2; SPT12.
Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Application Dilution
WB	1:300-5000