Phospho-CDK1/2 (Thr14) Recombinant Antibody

Name: Phospho-CDK1/2 (Thr14) Recombinant Antibody
Brand: Bioss
SKU: bsm-61198R
Price: 490.0 USD
Availability: InStock

$25 ug total protein per lane of various lysates (see on figure) probed with Phospho-CDK1\/2 (Thr14) monoclonal antibody, unconjugated (bsm-61198R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with Phospho-CDK1\/2 (Thr14) monoclonal antibody, unconjugated (bsm-61198R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

$Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Mouse Thymus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Rat Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

$Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.$

Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Phospho-CDK1\/2 (Thr14) Monoclonal Antibody, Unconjugated(bsm-61198R) at 1:200 overnight at 4\u00b0C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

Applications

WB
IHC-P
IHC-F
IF(IHC-P)
IP

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61198r-100uL
Product Name	Phospho-CDK1/2 (Thr14) Recombinant Antibody
Applications	WB IHC-P IHC-F IF(IHC-P) IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CDK1 around the phosphorylation site of T14
Modification Site	Thr14
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	983
Swiss Prot	P06493,P24941
Subcellular location	Cytoplasm, Nucleus
Synonyms	CDC28; CDC2A; CDK1; Cell division control protein 2 homolog; Cyclin-dependent kinase 1; MPF; kinase Cdc2; p34 protein kinase.
Background	Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition via association with multiple interphase.
Application Dilution
WB	1:1000-2000
IHC-P	IHC-P1:100-500
IHC-F	IHC-F1:100-500
IF(IHC-P)	IFIHC-P1:100-500
IP	IP1:20-50

Frequently asked questions

Which applications is Phospho-CDK1/2 (Thr14) Recombinant Antibody (bsm-61198R) validated for, and at what dilution?

bsm-61198R is validated for WB(WB) (1:1000-2000); IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-CDK1/2 (Thr14) Recombinant Antibody (bsm-61198R) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61198R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise Phospho-CDK1/2 (Thr14) Recombinant Antibody (bsm-61198R)?

KLH conjugated synthetic peptide derived from human CDK1 around the phosphorylation site of T14

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Phospho-CDK1/2 (Thr14) Recombinant Antibody

Applications

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Reactivity

BiossPM Rainbow Protein Marker (10~180 kDa)

BiossPM Rainbow Protein Marker (10~245 kDa)

BiossPM Rainbow Protein Marker (10~310 kDa)

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