Help

Phospho-CDK1/2 (Thr14) Recombinant Antibody, AbBy Fluor-405 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61198r-bf405
Product Name Phospho-CDK1/2 (Thr14) Recombinant Antibody, AbBy Fluor-405 Conjugated
Applications
WB IF(IHC-P)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CDK1 around the phosphorylation site of T14
Modification Site Thr14
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 983
Swiss Prot P06493,P24941
Subcellular location Cytoplasm, Nucleus
Synonyms CDC28; CDC2A; CDK1; Cell division control protein 2 homolog; Cyclin-dependent kinase 1; MPF; kinase Cdc2; p34 protein kinase.
Background Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition via association with multiple interphase.
Application Dilution
WB 1:1000-2000
IF(IHC-P) IFIHC-P1:100-500

Frequently asked questions

Which applications is Phospho-CDK1/2 (Thr14) Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61198R-BF405) validated for, and at what dilution?

bsm-61198R-BF405 is validated for WB(WB) (1:1000-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-CDK1/2 (Thr14) Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61198R-BF405) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61198R-BF405 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-CDK1/2 (Thr14) Recombinant Antibody, AbBy Fluor-405 Conjugated (bsm-61198R-BF405)?

KLH conjugated synthetic peptide derived from human CDK1 around the phosphorylation site of T14

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bsm-61198R-BF405)?

AbBy Fluor-405 on bsm-61198R-BF405: excitation / emission 401nm/421nm.