Phospho-CDK1/2 (Thr14) Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61198r-rbitc |
| Product Name | Phospho-CDK1/2 (Thr14) Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CDK1 around the phosphorylation site of T14 |
| Modification Site | Thr14 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 983 |
| Swiss Prot | P06493,P24941 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CDC28; CDC2A; CDK1; Cell division control protein 2 homolog; Cyclin-dependent kinase 1; MPF; kinase Cdc2; p34 protein kinase. |
| Background | Plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition via association with multiple interphase. |
| Application Dilution | |
| WB | 1:1000-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
Frequently asked questions
Which applications is Phospho-CDK1/2 (Thr14) Recombinant Antibody, RBITC Conjugated (bsm-61198R-RBITC) validated for, and at what dilution?
bsm-61198R-RBITC is validated for WB(WB) (1:1000-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-CDK1/2 (Thr14) Recombinant Antibody, RBITC Conjugated (bsm-61198R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61198R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-CDK1/2 (Thr14) Recombinant Antibody, RBITC Conjugated (bsm-61198R-RBITC)?
KLH conjugated synthetic peptide derived from human CDK1 around the phosphorylation site of T14
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-61198R-RBITC)?
RBITC on bsm-61198R-RBITC: excitation / emission 570nm/595nm.