RhoA/B/C Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61200r-rbitc-100uL |
| Product Name | RhoA/B/C Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RhoA |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 387 |
| Swiss Prot | P61586,P62745,P08134 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ARH12, ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC. |
| Background | RhoA , RhoB , RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function. |
| Application Dilution | |
| WB | 1:1000-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is RhoA/B/C Recombinant Antibody, RBITC Conjugated (bsm-61200R-RBITC) validated for, and at what dilution?
bsm-61200R-RBITC is validated for WB(WB) (1:1000-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RhoA/B/C Recombinant Antibody, RBITC Conjugated (bsm-61200R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61200R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RhoA/B/C Recombinant Antibody, RBITC Conjugated (bsm-61200R-RBITC)?
KLH conjugated synthetic peptide derived from human RhoA
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-61200R-RBITC)?
RBITC on bsm-61200R-RBITC: excitation / emission 570nm/595nm.