HP1 alpha Recombinant Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61202r-biotin-100uL |
| Product Name | HP1 alpha Recombinant Antibody, Biotin Conjugated |
| Applications |
WB
IHC-P
IHC-F
IP
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CBX5 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 23468 |
| Swiss Prot | P45973 |
| Subcellular location | Nucleus |
| Synonyms | CBX5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha. |
| Background | Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. |
| Application Dilution | |
| WB | 1:500-2000 |
| IHC-P | IHC-P1:100-500 |
| IHC-F | IHC-F1:100-500 |
| IP | IP1:20-50 |
Frequently asked questions
Which applications is HP1 alpha Recombinant Antibody, Biotin Conjugated (bsm-61202R-Biotin) validated for, and at what dilution?
bsm-61202R-Biotin is validated for WB(WB) (1:500-2000); IHC-P(IHC-P) (1:100-500); IHC-F(IHC-F) (1:100-500); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HP1 alpha Recombinant Antibody, Biotin Conjugated (bsm-61202R-Biotin) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61202R-Biotin be stored?
Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HP1 alpha Recombinant Antibody, Biotin Conjugated (bsm-61202R-Biotin)?
KLH conjugated synthetic peptide derived from human CBX5