HP1 alpha Recombinant Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61202r-percp-100uL |
| Product Name | HP1 alpha Recombinant Antibody, PerCP Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CBX5 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23468 |
| Swiss Prot | P45973 |
| Subcellular location | Nucleus |
| Synonyms | CBX5; Antigen p25; Heterochromatin protein 1 homolog alpha; HP1 alpha. |
| Background | Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1μg/Test |
| IF(IHC-P) | IFIHC-P1:100-500 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is HP1 alpha Recombinant Antibody, PerCP Conjugated (bsm-61202R-PerCP) validated for, and at what dilution?
bsm-61202R-PerCP is validated for WB(WB) (1:500-2000); FCM(FCM) (1μg/Test); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HP1 alpha Recombinant Antibody, PerCP Conjugated (bsm-61202R-PerCP) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61202R-PerCP be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HP1 alpha Recombinant Antibody, PerCP Conjugated (bsm-61202R-PerCP)?
KLH conjugated synthetic peptide derived from human CBX5
What are the excitation and emission wavelengths of the PerCP conjugate (bsm-61202R-PerCP)?
PerCP on bsm-61202R-PerCP: excitation / emission 482nm/677nm.