ASH2L Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with ASH2L monoclonal antibody, unconjugated (bsm-61210R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ASH2L monoclonal antibody, unconjugated (bsm-61210R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IHC-P
IHC-F
IF
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61210r-TR
Product Name	ASH2L Recombinant Antibody
Applications	WB, IHC-P, IHC-F, IF, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ASH2L
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9070
Swiss Prot	Q9UBL3
Subcellular location	Nucleus
Synonyms	ASH 2; ASH2; ASH2 LIKE; ASH2 like protein; ASH2-like protein; Ash2l; ASH2L1; ASH2L2; Bre 2; Bre2.
Background	Transcriptional regulator.Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters.Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	1:100-500
IF
ICC/IF	1:50-100