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Dnmt3a Recombinant Antibody, AbBy Fluor-680 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61213r-bf680
Product Name Dnmt3a Recombinant Antibody, AbBy Fluor-680 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation AbBy Fluor-680
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DNMT3A
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1788
Swiss Prot Q9Y6K1
Subcellular location Cytoplasm, Nucleus
Synonyms DNMT3A2; M.HsaIIIA; Dnmt3a; MCMT; DNA methyltransferase 3a.
Background Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development.DNA methylation is coordinated with methylation of histones.
Application Dilution
WB 1:500-2000
FCM FCM1ug/Test
IF(IHC-P) IFIHC-P1:100-500
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is Dnmt3a Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61213R-BF680) validated for, and at what dilution?

bsm-61213R-BF680 is validated for WB(WB) (1:500-2000); FCM(FCM) (1ug/Test); IF(IHC-P)(IF(IHC-P)) (1:100-500); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Dnmt3a Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61213R-BF680) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61213R-BF680 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Dnmt3a Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61213R-BF680)?

KLH conjugated synthetic peptide derived from human DNMT3A

What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-61213R-BF680)?

AbBy Fluor-680 on bsm-61213R-BF680: excitation / emission 679nm/702nm.