DGCR8 Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with DGCR8 monoclonal antibody, unconjugated (bsm-61214R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with DGCR8 monoclonal antibody, unconjugated (bsm-61214R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
IF(ICC)
IP

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61214r-TR
Product Name	DGCR8 Recombinant Antibody
Applications	WB IF(ICC) IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human DGCR8
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	54487
Swiss Prot	Q8WYQ5
Subcellular location	Nucleus
Synonyms	DGCR8; DGCRK6; Gy1 ; C22orf12; D16Wis2; pasha.
Background	Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus.
Application Dilution
WB	1:500-2000
IF(ICC)	IFICC1:50-200
IP	IP1:20-50