LIN28B Recombinant Antibody
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61215r-20uL |
| Product Name | LIN28B Recombinant Antibody |
| Applications |
WB
FCM
IF(ICC)
IP
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Lin 28B |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 389421 |
| Swiss Prot | Q6ZN17 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CSDD2; FLJ16517; LIN28B; Protein lin-28 homolog B. |
| Background | Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:50-200 |
| IP | IP1:20-50 |
Frequently asked questions
Which applications is LIN28B Recombinant Antibody (bsm-61215R) validated for, and at what dilution?
bsm-61215R is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LIN28B Recombinant Antibody (bsm-61215R) react with?
Tested reactivity: Human.
How should bsm-61215R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
What immunogen was used to raise LIN28B Recombinant Antibody (bsm-61215R)?
KLH conjugated synthetic peptide derived from human Lin 28B