mGluR5 Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61218r-bf647-100uL |
| Product Name | mGluR5 Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human mGluR5 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2915 |
| Swiss Prot | P41594 |
| Subcellular location | Cell membrane |
| Synonyms | GPRC1E; GRM5; Metabotropic glutamate receptor 5 precursor; MGLUR5; MGR5. |
| Background | G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity. |
| Application Dilution | |
| FCM | 1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is mGluR5 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61218R-BF647) validated for, and at what dilution?
bsm-61218R-BF647 is validated for FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does mGluR5 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61218R-BF647) react with?
Tested reactivity: Mouse, Rat.
How should bsm-61218R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise mGluR5 Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61218R-BF647)?
KLH conjugated synthetic peptide derived from human mGluR5
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-61218R-BF647)?
AbBy Fluor-647 on bsm-61218R-BF647: excitation / emission 650nm/665nm.