EEA1 Recombinant Antibody

$Western blot analysis of (1) HeLa cell lysate; (2) NIH\/3T3 cell lysate. Using EEA1 (bsm-61222R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of (1) HeLa cell lysate; (2) NIH\/3T3 cell lysate. Using EEA1 (bsm-61222R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
ICC/IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61222r
Product Name	EEA1 Recombinant Antibody
Applications	WB, ICC/IF
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Early endosome antigen 1
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8411
Swiss Prot	Q15075
Subcellular location	Cytoplasm, Cell membrane
Synonyms	Early endosome antigen 1; EEA1; MST105; ZFYVE2.
Background	Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Application Dilution
WB	1:300-5000
ICC/IF	1:50-100