PP2A alpha/beta Recombinant Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61223r-percp-Cy5.5-100uL |
| Product Name | PP2A alpha/beta Recombinant Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PP2A alpha |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5516 |
| Swiss Prot | P62714,P67775 |
| Synonyms | PP2A alpha; PP2A beta; Replication protein C; PPP2CA; PPP2CB. |
| Background | Protein phosphatase type 2A (PP2A) is an essential protein serine/threonine phosphatase that is conserved in all eukaryotes. PP2A is a key enzyme within various signal transduction pathways as it regulates fundamental cellular activities such as DNA replication, transcription, translation, metabolism, cell cycle progression, cell division, apoptosis and development. The core enzyme consists of catalytic C and regulatory A (or PR65) subunits, with each subunit represented by ŚÁ and ŚÂ isoforms. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is PP2A alpha/beta Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-61223R-PERCP-CY5-5) validated for, and at what dilution?
BSM-61223R-PERCP-CY5-5 is validated for WB (1:300-5000); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PP2A alpha/beta Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-61223R-PERCP-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BSM-61223R-PERCP-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PP2A alpha/beta Recombinant Antibody, PerCP-Cy5.5 Conjugated (BSM-61223R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human PP2A alpha
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BSM-61223R-PERCP-CY5-5)?
PerCP-Cy5.5 on BSM-61223R-PERCP-CY5-5: excitation / emission 482nm/694nm.