Acetyl CoA carboxylase Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61228r-cy3 |
| Product Name | Acetyl CoA carboxylase Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Acetyl CoA carboxylase |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | 1 mg/ml |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 32 |
| Swiss Prot | O00763 |
| Subcellular location | Cell membrane |
| Synonyms | ACAC; ACACA; ACACB; ACC; ACC-alpha; ACC1; ACC2; ACCA; ACCB; Acetyl-CoA carboxylase 1; Biotin carboxylase. |
| Background | ACC1 a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCŚÁ (ACC1) is the predominant isoform found in liver, adipocytes, and mammary gland, while the 280 kDa ACCŚÂ (ACC2) is the major isoform in skeletal muscle and heart. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-200 |
| IF(IHC-P) | IFIHC-P1:200-800 |
| IF | |
Frequently asked questions
Which applications is Acetyl CoA carboxylase Recombinant Antibody, Cy3 Conjugated (bsm-61228R-Cy3) validated for, and at what dilution?
bsm-61228R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-200); IF(IHC-P)(IF(IHC-P)) (1:200-800); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Acetyl CoA carboxylase Recombinant Antibody, Cy3 Conjugated (bsm-61228R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61228R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Acetyl CoA carboxylase Recombinant Antibody, Cy3 Conjugated (bsm-61228R-Cy3)?
KLH conjugated synthetic peptide derived from human Acetyl CoA carboxylase
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61228R-Cy3)?
Cy3 on bsm-61228R-Cy3: excitation / emission 512,550nm/570,615nm.