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GOLPH2 Recombinant Antibody, Cy3 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-61229r-cy3-100uL
Product Name GOLPH2 Recombinant Antibody, Cy3 Conjugated
Applications
WB FCM IF(IHC-P) IF(ICC)
Reactivity Human
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Golgi membrane protein 1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 51280
Swiss Prot Q8NBJ4
Subcellular location Extracellular, Cytoplasm, Cell membrane
Synonyms GOLPH2; GP73; GOLM1; Golgi protein 73kD; C9orf155.
Background The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:50-200
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is GOLPH2 Recombinant Antibody, Cy3 Conjugated (bsm-61229R-Cy3) validated for, and at what dilution?

bsm-61229R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GOLPH2 Recombinant Antibody, Cy3 Conjugated (bsm-61229R-Cy3) react with?

Tested reactivity: Human.

How should bsm-61229R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GOLPH2 Recombinant Antibody, Cy3 Conjugated (bsm-61229R-Cy3)?

KLH conjugated synthetic peptide derived from human Golgi membrane protein 1

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61229R-Cy3)?

Cy3 on bsm-61229R-Cy3: excitation / emission 512,550nm/570,615nm.