DAXX Recombinant Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61231r-rbitc-100uL |
| Product Name | DAXX Recombinant Antibody, RBITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DAXX |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1616 |
| Swiss Prot | Q9UER7 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Death domain-associated protein 6; Daxx; hDaxx; Fas death domain-associated protein; ETS1-associated protein 1; EAP1; DAXX; BING2; DAP6. |
| Background | Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is DAXX Recombinant Antibody, RBITC Conjugated (bsm-61231R-RBITC) validated for, and at what dilution?
bsm-61231R-RBITC is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DAXX Recombinant Antibody, RBITC Conjugated (bsm-61231R-RBITC) react with?
Tested reactivity: Human.
How should bsm-61231R-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DAXX Recombinant Antibody, RBITC Conjugated (bsm-61231R-RBITC)?
KLH conjugated synthetic peptide derived from human DAXX
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-61231R-RBITC)?
RBITC on bsm-61231R-RBITC: excitation / emission 570nm/595nm.