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Phospho-IRE1 (Ser724) Recombinant Antibody, AbBy Fluor-680 Conjugated

Applications

  • WB

Reactivity

  • Human
Overview
Catalog # bsm-61238r-bf680
Product Name Phospho-IRE1 (Ser724) Recombinant Antibody, AbBy Fluor-680 Conjugated
Applications
WB
Reactivity Human
Specifications
Conjugation AbBy Fluor-680
Host Rabbit
Source KLH conjugated synthetic peptide derived from human IRE1 alpha around the phosphorylation site of S724
Modification Site Ser724
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2081
Swiss Prot O75460
Subcellular location Cytoplasm, Cell membrane
Synonyms ERN1, ER to nucleus signalling 1, Inositol-requiring enzyme 1, Inositol-requiring protein 1, Ire1-alpha, IRE1a, HIRE1p, IRE1, Inositol-requiring 1, IRE1P.
Background Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR).
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Phospho-IRE1 (Ser724) Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61238R-BF680) validated for, and at what dilution?

bsm-61238R-BF680 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Phospho-IRE1 (Ser724) Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61238R-BF680) react with?

Tested reactivity: Human.

How should bsm-61238R-BF680 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Phospho-IRE1 (Ser724) Recombinant Antibody, AbBy Fluor-680 Conjugated (bsm-61238R-BF680)?

KLH conjugated synthetic peptide derived from human IRE1 alpha around the phosphorylation site of S724

What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bsm-61238R-BF680)?

AbBy Fluor-680 on bsm-61238R-BF680: excitation / emission 679nm/702nm.