ERR-alpha Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with ERR-alpha monoclonal antibody, unconjugated (bsm-61241R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with ERR-alpha monoclonal antibody, unconjugated (bsm-61241R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61241r
Product Name	ERR-alpha Recombinant Antibody
Applications	WB FCM IP
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human ERR alpha
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2101
Swiss Prot	P11474
Subcellular location	Nucleus
Synonyms	ESRRA; ERRa; ERR1; HERR1; NR3B1; Steroid hormone receptor ERR1; ERR-alpha; ERRalpha; ESRL1; Estrogen receptor-like 1; Estrogen-related receptor alpha.
Background	Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Induces the expression of PERM1 in the skeletal muscle.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100
IP	IP1:20-50