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SERCA1 Recombinant Antibody, Cy3 Conjugated

Applications

  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-61247r-cy3
Product Name SERCA1 Recombinant Antibody, Cy3 Conjugated
Applications
IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human SERCA1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 487
Swiss Prot O14983
Subcellular location Cytoplasm, Cell membrane
Synonyms ATP2A; ATP2A1; SERCA1.
Background Key regulator of striated muscle performance by acting as the major Ca2+ ATPase responsible for the reuptake of cytosolic Ca2+ into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen.Contributes to calcium sequestration involved in muscular excitation/contraction.
Application Dilution
IF(ICC) 1:50-200

Frequently asked questions

Which applications is SERCA1 Recombinant Antibody, Cy3 Conjugated (bsm-61247R-Cy3) validated for, and at what dilution?

bsm-61247R-Cy3 is validated for IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does SERCA1 Recombinant Antibody, Cy3 Conjugated (bsm-61247R-Cy3) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-61247R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise SERCA1 Recombinant Antibody, Cy3 Conjugated (bsm-61247R-Cy3)?

KLH conjugated synthetic peptide derived from human SERCA1

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61247R-Cy3)?

Cy3 on bsm-61247R-Cy3: excitation / emission 512,550nm/570,615nm.