NLRP3 Recombinant Antibody

$4% Paraformaldehyde-fixed THP-1(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NLRP3) monoclonal Antibody, unconjugated (bsm-61254R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed THP-1(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (NLRP3) monoclonal Antibody, unconjugated (bsm-61254R) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-FITC) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

$RAW264.7 (M) cells were treated with or without LPS (10 \u00b5g\/ml) for 8 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with NLRP3 monoclonal antibody, unconjugated (bsm-61254R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

RAW264.7 (M) cells were treated with or without LPS (10 \u00b5g\/ml) for 8 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with NLRP3 monoclonal antibody, unconjugated (bsm-61254R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

Reactivity

Mouse

Predicted Reactivity

Human
Rat

Overview
Catalog #	bsm-61254r
Product Name	NLRP3 Recombinant Antibody
Applications	WB
Reactivity	Mouse
Predicted Reactivity	Human, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	A synthesized peptide derived from human NLRP3
Clonality	Recombinant
Clone #	1F5
Isotype	IgG
Concentration	1mg/ml
Purification	purified by Protein A.
Storage Buffer	10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% Proclin300 and 50% glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	114548
Swiss Prot	Q96P20
Subcellular location	Cytoplasm
Synonyms	AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; DFNA34; FCAS; FCAS1; FCU; KEFH; MWS; NALP3; PYPAF1; AII/AVP; Mmig1; NLRP3_HUMAN; NLRP3; Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1 (CLR1.1); Cold-induced autoinflammatory syndrome 1 protein; Cryopyrin; PYRIN-containing APAF1-like protein 1; 3.6.4.-; NLRP3_MOUSE; Cold autoinflammatory syndrome 1 protein homolog; Mast cell maturation-associated-inducible protein 1; NLR family pyrin domain containing 3; cold autoinflammatory syndrome 1; deafness, autosomal dominant 34; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3
Background	Sensor component of the NLRP3 inflammasome, which mediates inflammasome activation in response to defects in membrane integrity, leading to secretion of inflammatory cytokines IL1B and IL18 and pyroptosis.
Application Dilution
WB	1:500-2000