LSP1 Recombinant Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61255r-bf594-100uL |
| Product Name | LSP1 Recombinant Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LSP1 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4046 |
| Swiss Prot | P33241 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Lymphocyte-specific protein 1; Protein pp52; 52 kDa phosphoprotein; Lymphocyte-specific antigen WP34; 47 kDa actin-binding protein. |
| Background | LSP1 is a 52 kDa leukocyte-specific protein expressed in B cells, T cells, macrophages and neutrophils. The protein binds F-actin through its C-terminal actin-binding repeat and regulates cytoskeletal-related cellular movement such as polarization, chemotaxis and locomotion. LSP1 is a phospho-protein and has been identified as a major substrate of MAP kinase and PKC in lymphocytes and neutrophils. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(IHC-P) | IFIHC-P1:50-200 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is LSP1 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-61255R-BF594) validated for, and at what dilution?
bsm-61255R-BF594 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:50-200); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LSP1 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-61255R-BF594) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61255R-BF594 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LSP1 Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-61255R-BF594)?
KLH conjugated synthetic peptide derived from human LSP1
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bsm-61255R-BF594)?
AbBy Fluor-594 on bsm-61255R-BF594: excitation / emission 590nm/617nm.