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RALBP1 Recombinant Antibody, Cy3 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61265r-cy3
Product Name RALBP1 Recombinant Antibody, Cy3 Conjugated
Applications
WB FCM IF(IHC-P)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RalBP1
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10928
Swiss Prot Q15311
Subcellular location Cytoplasm
Synonyms RALBP1; RIP1; RLIP1; RLIP76.
Background Multifunctional protein that functions as a downstream effector of RALA and RALB.As a GTPase-activating protein/GAP can inactivate CDC42 and RAC1 by stimulating their GTPase activity.As part of the Ral signaling pathway, may also regulate ligand-dependent EGF and insulin receptors-mediated endocytosis.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(IHC-P) IFIHC-P1:100-500

Frequently asked questions

Which applications is RALBP1 Recombinant Antibody, Cy3 Conjugated (bsm-61265R-Cy3) validated for, and at what dilution?

bsm-61265R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RALBP1 Recombinant Antibody, Cy3 Conjugated (bsm-61265R-Cy3) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61265R-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RALBP1 Recombinant Antibody, Cy3 Conjugated (bsm-61265R-Cy3)?

KLH conjugated synthetic peptide derived from human RalBP1

What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61265R-Cy3)?

Cy3 on bsm-61265R-Cy3: excitation / emission 512,550nm/570,615nm.