RAD18 Recombinant Antibody

$Western blot analysis of HeLa cell lysate. Using RAD18 (bsm-61274R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of HeLa cell lysate. Using RAD18 (bsm-61274R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

WB
Flow-Cyt

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61274r
Product Name	RAD18 Recombinant Antibody
Applications	WB, Flow-Cyt
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RAD18
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	56852
Swiss Prot	Q9NS91
Synonyms	E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; RAD18; RAD18, S. cerevisiae, homolog; RING finger protein 73; RNF73.
Background	E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
Application Dilution
WB	1:300-5000
Flow-Cyt	1ug/Test