RAD18 Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61274r-bf488 |
| Product Name | RAD18 Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human RAD18 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 56852 |
| Swiss Prot | Q9NS91 |
| Synonyms | E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; RAD18; RAD18, S. cerevisiae, homolog; RING finger protein 73; RNF73. |
| Background | E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is RAD18 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61274R-BF488) validated for, and at what dilution?
bsm-61274R-BF488 is validated for WB (1:300-5000); Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does RAD18 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61274R-BF488) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61274R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise RAD18 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61274R-BF488)?
KLH conjugated synthetic peptide derived from human RAD18
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-61274R-BF488)?
AbBy Fluor-488 on bsm-61274R-BF488: excitation / emission 499nm/519nm.