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BPI Recombinant Antibody, AbBy Fluor-594 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-61275r-bf594-100uL
Product Name BPI Recombinant Antibody, AbBy Fluor-594 Conjugated
Applications
WB FCM IF(ICC)
Reactivity Human
Specifications
Conjugation AbBy Fluor-594
Host Rabbit
Source KLH conjugated synthetic peptide derived from human BPI
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 671
Swiss Prot P17213
Subcellular location Cytoplasm, Secreted, Cell membrane
Synonyms rBPI; BPIFD1; CAP 57; BPI; Bactericidal permeability-increasing protein.
Background The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is BPI Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-61275R-BF594) validated for, and at what dilution?

bsm-61275R-BF594 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BPI Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-61275R-BF594) react with?

Tested reactivity: Human.

How should bsm-61275R-BF594 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BPI Recombinant Antibody, AbBy Fluor-594 Conjugated (bsm-61275R-BF594)?

KLH conjugated synthetic peptide derived from human BPI

What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bsm-61275R-BF594)?

AbBy Fluor-594 on bsm-61275R-BF594: excitation / emission 590nm/617nm.

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