Smac Recombinant Antibody, PE-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61284r-pe-cy5-100uL |
| Product Name | Smac Recombinant Antibody, PE-Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DIABLO |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | SMAC; DFNA64; DIABLO; SMAC3. |
| Background | Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
Frequently asked questions
Which applications is Smac Recombinant Antibody, PE-Cy5 Conjugated (bsm-61284R-PE-Cy5) validated for, and at what dilution?
bsm-61284R-PE-Cy5 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Smac Recombinant Antibody, PE-Cy5 Conjugated (bsm-61284R-PE-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61284R-PE-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Smac Recombinant Antibody, PE-Cy5 Conjugated (bsm-61284R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human DIABLO
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bsm-61284R-PE-Cy5)?
PE-Cy5 on bsm-61284R-PE-Cy5: excitation / emission 488nm/670nm.