MAP3K2 Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61285r-cy3-100uL |
| Product Name | MAP3K2 Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF
FLOW-CYT
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MEKK2 |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10746 |
| Swiss Prot | Q9Y2U5 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | MAP3K2; MAP/ERK kinase kinase 2; MAPK/ERK kinase kinase 2; MAPKKK2; MEK kinase 2; MEKK 2; MEKK2; MEKK2B. |
| Background | Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7.Plays a role in caveolae kiss-and-run dynamics. |
| Application Dilution | |
| WB | 1:500-2000 |
| IF | |
| Flow-Cyt | 1ug/Test |
Frequently asked questions
Which applications is MAP3K2 Recombinant Antibody, Cy3 Conjugated (bsm-61285R-Cy3) validated for, and at what dilution?
bsm-61285R-Cy3 is validated for WB (1:500-2000); IF; Flow-Cyt (1ug/Test). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MAP3K2 Recombinant Antibody, Cy3 Conjugated (bsm-61285R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61285R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MAP3K2 Recombinant Antibody, Cy3 Conjugated (bsm-61285R-Cy3)?
KLH conjugated synthetic peptide derived from human MEKK2
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61285R-Cy3)?
Cy3 on bsm-61285R-Cy3: excitation / emission 512,550nm/570,615nm.