ILK Recombinant Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61287r-bf647-100uL |
| Product Name | ILK Recombinant Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ILK |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3611 |
| Swiss Prot | Q13418 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | ILK; DKFZp686F1765; P59; ILK1; Integrin linked protein kinas. |
| Background | Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
| IF(ICC) | IFICC1:50-200 |
Frequently asked questions
Which applications is ILK Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61287R-BF647) validated for, and at what dilution?
bsm-61287R-BF647 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ILK Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61287R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61287R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ILK Recombinant Antibody, AbBy Fluor-647 Conjugated (bsm-61287R-BF647)?
KLH conjugated synthetic peptide derived from human ILK
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bsm-61287R-BF647)?
AbBy Fluor-647 on bsm-61287R-BF647: excitation / emission 650nm/665nm.