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ILK Recombinant Antibody, Cy7 Conjugated

Applications

  • WB
  • FCM
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bsm-61287r-cy7-100uL
Product Name ILK Recombinant Antibody, Cy7 Conjugated
Applications
WB FCM IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ILK
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3611
Swiss Prot Q13418
Subcellular location Cytoplasm, Cell membrane
Synonyms ILK; DKFZp686F1765; P59; ILK1; Integrin linked protein kinas.
Background Integrin-linked kinases (ILKs) couple integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. ILK functions as a scaffold bridging the extracellular matrix (ECM) and growth factor receptors to the actin cytoskeleton through interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is ILK Recombinant Antibody, Cy7 Conjugated (bsm-61287R-Cy7) validated for, and at what dilution?

bsm-61287R-Cy7 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ILK Recombinant Antibody, Cy7 Conjugated (bsm-61287R-Cy7) react with?

Tested reactivity: Human, Mouse, Rat.

How should bsm-61287R-Cy7 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ILK Recombinant Antibody, Cy7 Conjugated (bsm-61287R-Cy7)?

KLH conjugated synthetic peptide derived from human ILK

What are the excitation and emission wavelengths of the Cy7 conjugate (bsm-61287R-Cy7)?

Cy7 on bsm-61287R-Cy7: excitation / emission 743nm/767nm.