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CEBP alpha Recombinant Antibody, AbBy Fluor-488 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-61291r-bf488
Product Name CEBP alpha Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
WB FCM
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation AbBy Fluor-488
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CEBP alpha
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1050
Swiss Prot P49715
Subcellular location Nucleus
Synonyms C/EBP alpha; CCAAT/enhancer binding protein alpha; CEBPA.
Background Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes.During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100

Frequently asked questions

Which applications is CEBP alpha Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61291R-BF488) validated for, and at what dilution?

bsm-61291R-BF488 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CEBP alpha Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61291R-BF488) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-61291R-BF488 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CEBP alpha Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61291R-BF488)?

KLH conjugated synthetic peptide derived from human CEBP alpha

What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-61291R-BF488)?

AbBy Fluor-488 on bsm-61291R-BF488: excitation / emission 499nm/519nm.