CEBP alpha Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61291r-cy3 |
| Product Name | CEBP alpha Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CEBP alpha |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1050 |
| Swiss Prot | P49715 |
| Subcellular location | Nucleus |
| Synonyms | C/EBP alpha; CCAAT/enhancer binding protein alpha; CEBPA. |
| Background | Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes.During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
Frequently asked questions
Which applications is CEBP alpha Recombinant Antibody, Cy3 Conjugated (bsm-61291R-Cy3) validated for, and at what dilution?
bsm-61291R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CEBP alpha Recombinant Antibody, Cy3 Conjugated (bsm-61291R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bsm-61291R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CEBP alpha Recombinant Antibody, Cy3 Conjugated (bsm-61291R-Cy3)?
KLH conjugated synthetic peptide derived from human CEBP alpha
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61291R-Cy3)?
Cy3 on bsm-61291R-Cy3: excitation / emission 512,550nm/570,615nm.