NEDD4-2 Recombinant Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61294r-bf488-100uL |
| Product Name | NEDD4-2 Recombinant Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NEDD4L |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23327 |
| Swiss Prot | Q96PU5 |
| Subcellular location | Cytoplasm |
| Synonyms | E3 ubiquitin protein ligase NEDD4 like protein; KIAA0439; NEDD4 2; NEDD4 2c; Nedd4-2; NEDD4-2a; NEDD4b; NEDD4L; NEDL3; RSP5. |
| Background | E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins and is thereby implicated in the regulation of various signaling pathways including autophagy, innate immunity or DNA repair. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is NEDD4-2 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61294R-BF488) validated for, and at what dilution?
bsm-61294R-BF488 is validated for WB(WB) (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NEDD4-2 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61294R-BF488) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bsm-61294R-BF488 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NEDD4-2 Recombinant Antibody, AbBy Fluor-488 Conjugated (bsm-61294R-BF488)?
KLH conjugated synthetic peptide derived from human NEDD4L
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bsm-61294R-BF488)?
AbBy Fluor-488 on bsm-61294R-BF488: excitation / emission 499nm/519nm.