PKM2 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61301r-apc-cy5-100uL |
| Product Name | PKM2 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PKM |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5315 |
| Swiss Prot | P14618 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CTHBP; Cytosolic thyroid hormone binding protein; KPYM; OIP 3; Oip3; OIP3; OPA interacting protein 3; p58; PK Muscle type; muscle type; PK2; Pk3; PKM. |
| Background | Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PKM2 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61301R-APC-Cy5) validated for, and at what dilution?
bsm-61301R-APC-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PKM2 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61301R-APC-Cy5) react with?
Tested reactivity: Human, Mouse, Rat.
How should bsm-61301R-APC-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PKM2 Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61301R-APC-Cy5)?
KLH conjugated synthetic peptide derived from human PKM
What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-61301R-APC-Cy5)?
APC-Cy5 on bsm-61301R-APC-Cy5: excitation / emission 650nm/670nm.