INPP4B Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-61303r-cy3-100uL |
| Product Name | INPP4B Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human INPP4B |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 8821 |
| Swiss Prot | O15327 |
| Subcellular location | Cytoplasm |
| Synonyms | 4-bisphosphate 4-phosphatase; Inositol polyphosphate 4 phosphatase type II 105kDa; Inositol polyphosphate 4-phosphatase type II; Inpp4b; MGC132014; Type II inositol 3,4 bisphosphate 4 phosphatase; Type II inositol-3. |
| Background | Catalyzes the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-trisphosphate. Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles.The lipid phosphatase activity is critical for tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival |
| Application Dilution | |
| WB | 1:500-2000 |
| IF(IHC-P) | IFIHC-P1:100-500 |
Frequently asked questions
Which applications is INPP4B Recombinant Antibody, Cy3 Conjugated (bsm-61303R-Cy3) validated for, and at what dilution?
bsm-61303R-Cy3 is validated for WB(WB) (1:500-2000); IF(IHC-P)(IF(IHC-P)) (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does INPP4B Recombinant Antibody, Cy3 Conjugated (bsm-61303R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should bsm-61303R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise INPP4B Recombinant Antibody, Cy3 Conjugated (bsm-61303R-Cy3)?
KLH conjugated synthetic peptide derived from human INPP4B
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61303R-Cy3)?
Cy3 on bsm-61303R-Cy3: excitation / emission 512,550nm/570,615nm.