Argonaute 3/eIF2C3 Recombinant Antibody

$Western blot analysis of Human brain lysate. Using Argonaute 3\/eIF2C3 (bsm-61315R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n$

Western blot analysis of Human brain lysate. Using Argonaute 3\/eIF2C3 (bsm-61315R) monoclonal antibody at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.\r\n

Applications

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bsm-61315r
Product Name	Argonaute 3/eIF2C3 Recombinant Antibody
Applications	WB
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human eIF2C3
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	192669
Swiss Prot	Q9H9G7
Subcellular location	Cytoplasm
Synonyms	EIF2C3; Argonaute3; Argonaute 3; AGO3; EIF-2C 3; HAgo3; EIF2C 3; Protein argonaute-3.
Background	Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (siRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent siRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4.
Application Dilution
WB	1:300-5000