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Caspase 10 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-61328r-pe-cy3-100uL
Product Name Caspase 10 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated
Applications
WB FCM
Reactivity Human, Mouse
Specifications
Conjugation PE-Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Caspase 10
Clonality Monoclonal
Clone # Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 843
Swiss Prot Q92851
Subcellular location Cytoplasm
Synonyms Apoptotic protease Mch4; CASP10; Caspase 10; FLICE2; ICE like apoptotic protease 4; FADD-like ICE2.
Background Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates effector caspases CASP3, CASP4, CASP6, CASP7, CASP8 and CASP9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC.
Application Dilution
WB 1:300-5000
FCM 1:20-100

Frequently asked questions

Which applications is Caspase 10 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61328R-PE-Cy3) validated for, and at what dilution?

bsm-61328R-PE-Cy3 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Caspase 10 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61328R-PE-Cy3) react with?

Tested reactivity: Human, Mouse.

How should bsm-61328R-PE-Cy3 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Caspase 10 Recombinant Monoclonal Antibody, PE-Cy3 Conjugated (bsm-61328R-PE-Cy3)?

KLH conjugated synthetic peptide derived from human Caspase 10

What are the excitation and emission wavelengths of the PE-Cy3 conjugate (bsm-61328R-PE-Cy3)?

PE-Cy3 on bsm-61328R-PE-Cy3: excitation / emission 488nmn/578nm.