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HSPA14 Recombinant Antibody

25 ug total protein per lane of various lysates (see on figure) probed with HSPA14 monoclonal antibody, unconjugated (bsm-61329R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
25 ug total protein per lane of various lysates (see on figure) probed with HSPA14 monoclonal antibody, unconjugated (bsm-61329R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-HSPA14 antibody (bsm-61329R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-HSPA14 antibody (bsm-61329R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • WB
  • FCM
  • IP

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
Overview
Catalog # bsm-61329r-TR
Product Name HSPA14 Recombinant Antibody
Applications
WB FCM IP
Reactivity Human
Predicted Reactivity Mouse, Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Hsp70 14
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 51182
Swiss Prot Q0VDF9
Subcellular location Cytoplasm
Synonyms HSPA14; Heat shock protein HSP60; Heat shock 70 kDa protein 14; HSP70L1; HSP60; Heat shock 70kDa protein 14; HSP70-like protein 1; HSP70-4.
Background Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100
IP IP1:20-50

Frequently asked questions

Which applications is HSPA14 Recombinant Antibody (bsm-61329R) validated for, and at what dilution?

bsm-61329R is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100); IP(IP) (1:20-50). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HSPA14 Recombinant Antibody (bsm-61329R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.

How should bsm-61329R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol

What immunogen was used to raise HSPA14 Recombinant Antibody (bsm-61329R)?

KLH conjugated synthetic peptide derived from human Hsp70 14

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