CDC25C Recombinant Monoclonal Antibody, APC-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61331r-apc-cy5-100uL |
| Product Name | CDC25C Recombinant Monoclonal Antibody, APC-Cy5 Conjugated |
| Applications |
WB
FCM
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | APC-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CDC25C |
| Clonality | Monoclonal |
| Clone # | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 995 |
| Swiss Prot | P30307 |
| Subcellular location | Nucleus |
| Synonyms | CDC25; CDC25C; Cell division cycle 25 homolog C; MPIP3; PPP1R60. |
| Background | Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle.When phosphorylated, highly effective in activating G2 cells into prophase.Directly dephosphorylates CDK1 and activates its kinase activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CDC25C Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61331R-APC-Cy5) validated for, and at what dilution?
bsm-61331R-APC-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CDC25C Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61331R-APC-Cy5) react with?
Tested reactivity: Human.
How should bsm-61331R-APC-Cy5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CDC25C Recombinant Monoclonal Antibody, APC-Cy5 Conjugated (bsm-61331R-APC-Cy5)?
KLH conjugated synthetic peptide derived from human CDC25C
What are the excitation and emission wavelengths of the APC-Cy5 conjugate (bsm-61331R-APC-Cy5)?
APC-Cy5 on bsm-61331R-APC-Cy5: excitation / emission 650nm/670nm.