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CDC25C Recombinant Antibody, FITC Conjugated

Applications

  • WB
  • IF(ICC)

Reactivity

  • Human
Overview
Catalog # bsm-61331r-fitc-100uL
Product Name CDC25C Recombinant Antibody, FITC Conjugated
Applications
WB IF(ICC)
Reactivity Human
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CDC25C
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 995
Swiss Prot P30307
Subcellular location Nucleus
Synonyms CDC25; CDC25C; Cell division cycle 25 homolog C; MPIP3; PPP1R60.
Background Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle.When phosphorylated, highly effective in activating G2 cells into prophase.Directly dephosphorylates CDK1 and activates its kinase activity.
Application Dilution
WB 1:500-2000
IF(ICC) IFICC1:50-200

Frequently asked questions

Which applications is CDC25C Recombinant Antibody, FITC Conjugated (bsm-61331R-FITC) validated for, and at what dilution?

bsm-61331R-FITC is validated for WB(WB) (1:500-2000); IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CDC25C Recombinant Antibody, FITC Conjugated (bsm-61331R-FITC) react with?

Tested reactivity: Human.

How should bsm-61331R-FITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CDC25C Recombinant Antibody, FITC Conjugated (bsm-61331R-FITC)?

KLH conjugated synthetic peptide derived from human CDC25C

What are the excitation and emission wavelengths of the FITC conjugate (bsm-61331R-FITC)?

FITC on bsm-61331R-FITC: excitation / emission 494nm/518nm.