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CDC25B Recombinant Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
Overview
Catalog # bsm-61332r-bf750
Product Name CDC25B Recombinant Antibody, AbBy Fluor-750 Conjugated
Applications
WB FCM
Reactivity Human
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CDC25B
Clonality Recombinant
Isotype IgG
Concentration Lot dependent
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 994
Swiss Prot P30305
Subcellular location Cytoplasm
Synonyms CDC25HU2; CDC25M2; Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M-phase inducer phosphatase 2; MPIP2.
Background CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals.
Application Dilution
WB 1:500-2000
FCM FCM1:50-100

Frequently asked questions

Which applications is CDC25B Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-61332R-BF750) validated for, and at what dilution?

bsm-61332R-BF750 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CDC25B Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-61332R-BF750) react with?

Tested reactivity: Human.

How should bsm-61332R-BF750 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CDC25B Recombinant Antibody, AbBy Fluor-750 Conjugated (bsm-61332R-BF750)?

KLH conjugated synthetic peptide derived from human CDC25B

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bsm-61332R-BF750)?

AbBy Fluor-750 on bsm-61332R-BF750: excitation / emission 749nm/775nm.