CDC25B Recombinant Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-61332r-cy3 |
| Product Name | CDC25B Recombinant Antibody, Cy3 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CDC25B |
| Clonality | Recombinant |
| Isotype | IgG |
| Concentration | Lot dependent |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 994 |
| Swiss Prot | P30305 |
| Subcellular location | Cytoplasm |
| Synonyms | CDC25HU2; CDC25M2; Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M-phase inducer phosphatase 2; MPIP2. |
| Background | CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. |
| Application Dilution | |
| WB | 1:500-2000 |
| FCM | FCM1:50-100 |
Frequently asked questions
Which applications is CDC25B Recombinant Antibody, Cy3 Conjugated (bsm-61332R-Cy3) validated for, and at what dilution?
bsm-61332R-Cy3 is validated for WB(WB) (1:500-2000); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CDC25B Recombinant Antibody, Cy3 Conjugated (bsm-61332R-Cy3) react with?
Tested reactivity: Human.
How should bsm-61332R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CDC25B Recombinant Antibody, Cy3 Conjugated (bsm-61332R-Cy3)?
KLH conjugated synthetic peptide derived from human CDC25B
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-61332R-Cy3)?
Cy3 on bsm-61332R-Cy3: excitation / emission 512,550nm/570,615nm.