CDC45L Recombinant Antibody

$25 ug total protein per lane of various lysates (see on figure) probed with CDC45L monoclonal antibody, unconjugated (bsm-61334R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.$

25 ug total protein per lane of various lysates (see on figure) probed with CDC45L monoclonal antibody, unconjugated (bsm-61334R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Applications

WB
FCM
IF(ICC)

Reactivity

Human

Predicted Reactivity

Mouse
Rat

Overview
Catalog #	bsm-61334r
Product Name	CDC45L Recombinant Antibody
Applications	WB, FCM, IF(ICC)
Reactivity	Human
Predicted Reactivity	Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human CDC45
Clonality	Recombinant
Isotype	IgG
Concentration	Lot dependent
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS (pH 7.4), 1% BSA, 0.02% Proclin 300, and 50% Glycerol
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	8318
Swiss Prot	O75419
Subcellular location	Cytoplasm, Nucleus
Synonyms	CDC45L; CDC45L2; PORC-PI-1; CDC45.
Background	Required for initiation of chromosomal DNA replication. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built.
Application Dilution
WB	1:500-2000
FCM	FCM1:50-100
IF(ICC)	IFICC1:50-200